Multi-color retrograde tracing
Distinct frontal cortex neurons projecting to two downstream target regions (red and green).
Monosynaptic & cell-type specific rabies tracing
Presynaptic inputs from the basal forebrain to PV interneurons in the frontal cortex
Anterograde tracing
Midbrain neurons that receive frontal cortex inputs (magenta); GABA-containing neurons are shown in green.