Multi-color retrograde tracing

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Distinct frontal cortex neurons projecting to two downstream target regions (red and green).

 

Monosynaptic & cell-type specific rabies tracing

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Presynaptic inputs from the basal forebrain to PV interneurons in the frontal cortex

 

Anterograde tracing

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Midbrain neurons that receive frontal cortex inputs (magenta); GABA-containing neurons are shown in green.